ANTIBACTERIAL ACTIVITY OF ETHANOL EXTRACTS AND n-hexane OF (Centella asiatica (L.) Urb.) AGAINST THE Pseudomonas aeruginosa AND Bacillus subtilis
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Date
2020
Journal Title
Journal ISSN
Volume Title
Publisher
International Journal of Psychosocial Rehabilitation, Vol. 24, Issue 1
Abstract
Centella asiatica (L.) Urb.) Is a plant that has antifungal and antibacterial bioactive compounds.
Centella asiatica (L.) Urb.) Is one of the main herbs for treating skin problems, healing wounds and being an
antibacterial and antiviral agent. Centella asiatica (L.) Urb.) Leaf samples taken from the manoko bandung
plantation were extracted using 96% ethanol and n-hexane, the extract was thickened and a thick extract was
obtained which was then tested for its antibacterial activity. Antibacterial activity test uses the paper disk diffusion
method and tested against the bacteria Pseudomonas aeruginosa and Bacillus subtilis. The extract concentrations
used were 7.5%, 10%, 12.5% and 15%. The positive control used is tetracycline while the negative control used
is DMSO. The results showed that ethanol extract was effective in inhibiting the growth of Bacillus subtilis bacteria
with an average inhibition zone of 10.41 mm and included in the strong category. As for n-hexane, it is effective in
inhibiting the growth of Bacillus subtilis bacteria with inhibition zones 23.19 and categorized as very strong
inhibition. The ethanol and n-hexane extracts of Centella asiatica (L.) Urb.) Leaves are effective in inhibiting
bacteria from the gram positives group because gram positives have a simpler cell wall with a relatively large
number of peptidoglycan.
Description
Keywords
antibacterial, paper disk, Centella asiatica (L.) Urb.), B. Subtilis, P.aeruginosa