Browsing by Author "Naroeni, Aroem"

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    OPTIMIZATION OF IN HOUSE POLYMERASE CHAIN REACTION FOR HIGH RISK HUMAN PAPILLOMAVIRUS (HPV 18) E1 GENEBASED DETECTION
    (International Journal of Psychosocial Rehabilitation, Vol.24, Issue 01, 2020) Naroeni, Aroem; Arrasul, Lifda Nirmala Putri; Saraswati, Henny; Iskandar, Asep Deni
    Human Papillomavirus is a virus that can infect human and a causative agent for cervical cancer. This disease mostly caused by HPV type 16 and 18. Polymerase Chain Reaction (PCR) is one of the molecular biology method can be used to detect HPV in sample. Gen E1 in HPV genome has a role in virus replication and transcription, and relatively conserved. This gene can be used in HPV genotyping and detection. This research aim is to find the PCR optimal reaction for HPV type 18 detection. The E1 gene data from the National Center for Biotechnology Information (NCBI) was used in designing primer with Primer-BLAST NCBI. Selection of the primers was done by in silico analyzes using DINAmelt and Mfold. Optimization of annealing temperature and primer concentration was done for selected primers. The result showed that optimal annealing temperature is 59oC with optimal primer concentration is 600 nM.
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    OPTIMIZATION OF IN-HOUSE PCR ASSAY FOR HUMAN BCL-2 GENE
    (International Journal of Psychosocial Rehabilitation, Vol.24, Issue 01, 2020) Saraswati, Henny; Pulungan, Anita Nauli; Naroeni, Aroem; Fauzi, Esa
    BCL-2 gene is present on human chromosome 18 and contributes in regulating the mechanism of apoptotic cells. Mutations and overexpression of the BCL-2 gene are known to be associated with several cancers. Comprehensive research on the role of BCL-2 in the incidence of cancer is needed to understand course of the diseases and treatment. Polymerase Chain Reaction (PCR) method for copying the BCL-2 gene can be used in this research. Optimization of the in-house PCR reaction was carried out to determine the optimal conditions of the PCR reaction on the doubling of the BCL-2 gene. In-silico analysis of the primer design produces three pairs of primers used in the optimization process. The parameters used in this optimization are the annealing temperature and primer concentration used. Validation of the results of optimization of the PCR reaction was carried out by biopsy samples of tumor tissue of patients with cervical tissue abnormalities. The result showed that the best primer pairs of the reaction is primer C with optimal annealing temperature is 570C and 800 nM concentration.